Journal: Acta Pharmacologica Sinica

Article Title: Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

doi: 10.1038/aps.2012.129

Figure Lengend Snippet: Photomicrographs of representative iNOS-positive and 3-nitrotyrosine-positive stains in gingival tissue from patients with chronic periodontitis (immunohistochemical staining): (A) countless iNOS+ PMNs and monocytes (scale bar, 50 μm); (B) decreased iNOS+ cells in the SRP+placebo group (scale bar, 50 μm); (C) sparse iNOS+ cells in SRP+SDD group (scale bar, 50 μm); (D) countless 3NT+ PMNs and monocytes (scale bar, 100 μm); (E) decreased 3NT+ cells in the SRP+placebo group (scale bar, 50 μm); (F) sparse 3NT+ cells in the SRP+SDD group (scale bar, 50 μm). This figure is representative of 3 measurements (×400). iNOS+=induced nitric oxide synthase positive cells; 3NT+=3-nitrotyrosine positive cells; SRP=scaling and root planing; SDD=subantimicrobial dose of doxycycline.

Article Snippet: The following primary monoclonal antibodies were used: anti iNOS (R&D Systems, RD-MAB9502, MN, USA), anti 3-nitrotyrosine (3NT) (Novus Europe, KA0445, ABNOVA, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining

Journal: Acta Pharmacologica Sinica

Article Title: Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

doi: 10.1038/aps.2012.129

Figure Lengend Snippet: Periodontal immunohystochemical and serum nitrosative stress parameters of the patients.

Article Snippet: The following primary monoclonal antibodies were used: anti iNOS (R&D Systems, RD-MAB9502, MN, USA), anti 3-nitrotyrosine (3NT) (Novus Europe, KA0445, ABNOVA, Cambridge, UK).

Techniques:

Journal: Frontiers in Immunology

Article Title: LTr1 alleviates DSS-induced ulcerative colitis by modulating macrophages to inhibit M1 polarization and associated inflammatory responses

doi: 10.3389/fimmu.2025.1651922

Figure Lengend Snippet: LTr1 reduces DSS-induced macrophage infiltration and M1 Polarization. (A) The proportions of macrophages in the splenocytes and LNLs were determined by flow cytometry analysis. (B) Protein expression levels of iNOS and CD80 in macrophages of LNLs, along with the percentage of iNOS + or CD80 + macrophages as determined by flow cytometry. (C) mRNA expression levels of iNOS , CD80 , Arg1 , CD206 in colon tissue determined by quantitative RT-PCR. All experiments were performed with n = 5 mice per group and presented as means ± SD. Statistical analysis was performed using one-way ANOVA followed by LSD multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The following antibodies were used for flow cytometry: CD3-Brilliant Violet 510 (eBiosciences, Cat# 464882), CD11b-Brilliant Violet 421 (Biolegend, Cat# 101235), CD19-Brilliant Violet 650 (BD Biosciences, Cat# 563235), CD45-FITC (Biolegend, Cat# 103108), F4/80-PE (BD Biosciences, Cat# 565410). iNOS-APC (Miltenyibiotec, Cat# 130-116-423), CD80-Brilliant Violet 711 (Biolegend, Cat# 104743), CD206-PE/Cyanine7 (Biolegend, Cat# 141720).

Techniques: Flow Cytometry, Expressing, Quantitative RT-PCR

Journal: Frontiers in Immunology

Article Title: LTr1 alleviates DSS-induced ulcerative colitis by modulating macrophages to inhibit M1 polarization and associated inflammatory responses

doi: 10.3389/fimmu.2025.1651922

Figure Lengend Snippet: LTr1 directly acted on macrophages. (A) Cytotoxicity of LTr1 to RAW264.7 cell line was determined by CCK8 cell viability assay. (B) Protein expression levels of iNOS, CD80, and CD206 in RAW264.7 cell line was determined by flow cytometry. (C) ELISA analysis of IL-1β and IL-6 in cell culture medium. (D) mRNA expression levels of iNOS , IL-1β , and IL-6 in colon tissue determined by quantitative RT-PCR. Data were collected from at least three independent experiments and presented as means ± SD. All data statistical differences were evaluated using Permutation test and Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The following antibodies were used for flow cytometry: CD3-Brilliant Violet 510 (eBiosciences, Cat# 464882), CD11b-Brilliant Violet 421 (Biolegend, Cat# 101235), CD19-Brilliant Violet 650 (BD Biosciences, Cat# 563235), CD45-FITC (Biolegend, Cat# 103108), F4/80-PE (BD Biosciences, Cat# 565410). iNOS-APC (Miltenyibiotec, Cat# 130-116-423), CD80-Brilliant Violet 711 (Biolegend, Cat# 104743), CD206-PE/Cyanine7 (Biolegend, Cat# 141720).

Techniques: Viability Assay, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture, Quantitative RT-PCR

Journal: Cell reports

Article Title: Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetes

doi: 10.1016/j.celrep.2022.111011

Figure Lengend Snippet:

Article Snippet: anti-iNOS , Fluidigm , 3161011C.

Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Red Blood Cell Lysis, Reverse Transcription, Cell Isolation, Staining, RNA Sequencing, Software

Journal: Oncology Letters

Article Title: Overexpression of MTH1 and OGG1 proteins in ulcerative colitis-associated carcinogenesis

doi: 10.3892/ol.2018.8812

Figure Lengend Snippet: Antibodies used in immunohistochemical stain.

Article Snippet: Finally, sections were counterstained with Mayer's hematoxylin. table ft1 table-wrap mode="anchored" t5 Table I. caption a7 Antigen Primary antibodies Dilution Source Antigen retrieval iNOS nos typeII Mouse monoclonal 1:100 BD Biosciences, Franklin Lakes, NJ Microwaved for 30 min in target retrieval solution high pH (DAKO, Glostrup, Denmark) 8-OHdG N45.1 Mouse monoclonal 1:20 Japanese aging control institute, Shizuoka, Japan Proteinase K for 7 min incubate with 4N HCl for 20 min and with 50 mM Tris base for 5 min OGG1 NB100-106 Rabbit polyclonal 1:250 Novus Biologicals, Littleton, CO, Microwaved for 30 min in target retrieval solution high pH (DAKO, Glostrup, Denmark) MTH1 D-2 Rabbit polyclonal 1:400 Abcam, Cambridge, England Microwaved for 30 min in target retrieval solution high pH (DAKO, Glostrup, Denmark) MUTYH ab13698 Rabbit-polyclonal 1:50 Abcam, Cambridge, England Microwaved for 30 min in target retrieval solution high pH (DAKO, Glostrup, Denmark) p53 PAb1801 Mouse monoclonal 1:100 Oncogene Research Products, San Diego, CA.

Techniques: Immunohistochemical staining, Staining